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You are here:Home » Antibodies » Abs to Tubulin » Anti -Tubulin, beta 3 (Tubulin beta III, TUBB 3, TUBB3, Neuron Specific beta III Tubulin, beta-4, MC1R, Tubulin beta 4, TUBB 4)

Anti -Tubulin, beta 3 (Tubulin beta III, TUBB 3, TUBB3, Neuron Specific beta III Tubulin,
beta-4, MC1R, Tubulin beta 4, TUBB 4)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites E B IP IH IC
Class 3 beta tubulin (beta 3-tubulin) is a vertebrate tubulin Isotype specific to the neurons and mammalian testis cells, making it an ideal neuronal marker. Overexpression of class 3 beta tubulin is associated with the resistances of microtubule- targeted cancer drugs in lung cancer cell lines, breast cancer cell lines, and ovarian tumors.
Catalog #T9155-10
ApplicationsSuitable for use in Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot and ELISA. Other applications have not been tested.
Recommended DilutionImmunocytochemistry (Fixed): 1:100-1:500.
Immunohistochemistry: 1:20-1:100. Paraffin embedded, formalin fixed tissue. High temperature citrate buffer antigen retrieval is recommended with paraffin embedded tissue sections.
Western Blot: 1:1000.
Optimal dilutions to be determined by the researcher.
Positive Control: Brain tissue or neurons of any type.
Negative Control Non-neuronal tissue or Astrocytes.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2Q121
HostMouse
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in PBS, 1% BSA and 15mM sodium azide.
PurityAscites.
ImmunogenSynthetic peptide corresponding to aminno acids 443-450, ESESQGPK, of human class lll beta-tubulin. KLH conjugated. Epitope: C-terminus.
SpecificityRecognizes the beta III isoform of human tubulin which is specific for neurons. Species Crossreactivity: mouse, avian, porcine, rat, bovine, sheep and monkey. Does not identify beta tubulin found in glial cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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