Anti -Tubulin, beta 3 (Tubulin beta III, TUBB 3, TUBB3, Neuron Specific beta III Tubulin,
beta-4, MC1R, Tubulin beta 4, TUBB 4)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Ascites |
E B IP IH IC |
|
| Class 3 beta tubulin (beta 3-tubulin) is a vertebrate tubulin Isotype specific to the neurons and mammalian testis cells, making it an ideal neuronal marker. Overexpression of class 3 beta tubulin is associated with the resistances of microtubule- targeted cancer drugs in lung cancer cell lines, breast cancer cell lines, and ovarian tumors. | | | Catalog # | T9155-10 | | Applications | Suitable for use in Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot and ELISA. Other applications have not been tested. | | Recommended Dilution | Immunocytochemistry (Fixed): 1:100-1:500. | | Immunohistochemistry: 1:20-1:100. Paraffin embedded, formalin fixed tissue. High temperature citrate buffer antigen retrieval is recommended with paraffin embedded tissue sections. | | Western Blot: 1:1000. | | Optimal dilutions to be determined by the researcher. | | | | Positive Control: Brain tissue or neurons of any type. | | Negative Control | Non-neuronal tissue or Astrocytes. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 2Q121 | | Host | Mouse | | Source | Human | | Concentration | Not Determined | | Form | Supplied as a liquid in PBS, 1% BSA and 15mM sodium azide. | | Purity | Ascites. | | Immunogen | Synthetic peptide corresponding to aminno acids 443-450, ESESQGPK, of human class lll beta-tubulin. KLH conjugated. Epitope: C-terminus. | | Specificity | Recognizes the beta III isoform of human tubulin which is specific for neurons. Species Crossreactivity: mouse, avian, porcine, rat, bovine, sheep and monkey. Does not identify beta tubulin found in glial cells. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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