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You are here:Home » Antibodies » Abs to Receptors » Anti -Tumor Necrosis Factor Receptor 2, p75/p80 (TNFR2, CD120b) (Biotin)

Anti -Tumor Necrosis Factor Receptor 2, p75/p80 (TNFR2, CD120b) (Biotin)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH FC
TNF-RII is present on most cell types and is considered to be an important co-stimulator together with TNF-RI in cellular responses to TNF-alpha, in particular the transmembrane form of TNF-alpha. A dominant role of TNF-RII has been shown in thymocyte activation by TNF-alpha. The antibody is a non-agonistic receptor modulating antibody. It enhances in vitro TNF responses by increasing the affinity of the soluble form to TNF-alpha to TNF-RII.
Catalog #T9161-26E
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot and ELISA.
Other applications not tested.
Recommended DilutionImmunohistochemistry (frozen): 1:10
Flow Cytometry: 1:10
Western Blot: 1:10
Optimal dilutions to be determined by the researcher.
Recommended Positive ControlImmunohistochemistry: human lymphnodes
Flow Cytometry: PHA activated T cells.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No6A665
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, 0.02% sodium azide, 0.1% BSA. Labeled with Biotin.
PurityPurified IgG
ImmunogenHuman CD120b.
SpecificityReacts with the extracellular part of the TNF-RII. It also reacts with the soluble receptor. Reactivity with cellbound TNF-Receptor is minimally inhibited by high concentrations of TNF-alpha.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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