| Polyubiquitin chains linked from the Lys48 of one protein to the C-terminus of the other are the most common ubiquitin linkage. Lys48- linked ubiquitins are abundant in vivo and act as a universal signal for proteins targeted for proteosomal degradation. |
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| Catalog # | U1000-06P |
| Applications | Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry, Peptide Inhibition Assay and Immunocytochemistry. Other applications not tested. |
| Recommended Dilutions | Flow Cytometry: 1:700 permeabilized Jurkat cells |
| Western Blot: 1:1000-1:5000 |
| Immunoprecipitation: 10ul/100ug HeLa cell lysate |
| Immunohistochemistry: 1:100. Formalin fixed sections were heated in citrate buffer, pH 6. |
| Peptide Inhibition Assay: 1:1000 dilution with K48-multiubiquitin chains inhibited binding to HeLa cell lysate. |
| Immunocytochemistry: 1:500 |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1904-46E: IgG, H&L (HRP) Pab DkxRb |
| I1904-46K: IgG, H&L, X-Adsorbed (HRP) Pab DkxRb |
| Positive Control | Lys48 polyubiquitin chains |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG |
| Clone No | 9H284 |
| Host | Rabbit |
| Source | Human |
| Concentration | Not Determined |
| Form | Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Human di-ubiquitin linked at Lys48. |
| Specificity | Recognizes human ubiquitin linked at Lys48. Does not recognize ubiquitin linked at Lys63 or free ubiquitin. Species Crossreactivity: mouse, rabbit, and rat. Based on sequence homology, broad species crossreactivity is expected. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
