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You are here:Home » Antibodies » Abs to Ubiquitin » Anti -Ubiquitin (Lys48)

Anti -Ubiquitin (Lys48)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Affinity Purified B IP IH IC FC
Polyubiquitin chains linked from the Lys48 of one protein to the C-terminus of the other are the most common ubiquitin linkage. Lys48- linked ubiquitins are abundant in vivo and act as a universal signal for proteins targeted for proteosomal degradation.
Catalog #U1000-06P
ApplicationsSuitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry, Peptide Inhibition Assay and Immunocytochemistry. Other applications not tested.
Recommended DilutionsFlow Cytometry: 1:700 permeabilized Jurkat cells
Western Blot: 1:1000-1:5000
Immunoprecipitation: 10ul/100ug HeLa cell lysate
Immunohistochemistry: 1:100. Formalin fixed sections were heated in citrate buffer, pH 6.
Peptide Inhibition Assay: 1:1000 dilution with K48-multiubiquitin chains inhibited binding to HeLa cell lysate.
Immunocytochemistry: 1:500
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-46E: IgG, H&L (HRP) Pab DkxRb
I1904-46K: IgG, H&L, X-Adsorbed (HRP) Pab DkxRb
Positive ControlLys48 polyubiquitin chains
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS NumberN/A
Clone TypeMonoclonal
IsotypeIgG
Clone No9H284
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman di-ubiquitin linked at Lys48.
SpecificityRecognizes human ubiquitin linked at Lys48. Does not recognize ubiquitin linked at Lys63 or free ubiquitin. Species Crossreactivity: mouse, rabbit, and rat. Based on sequence homology, broad species crossreactivity is expected.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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