UGRP-2 is an 8kD member of the secretoglobulin family of molecules. It is synthesized as a 104aa precursor that contains a 21aa signal sequence and an 83aa mature region. There are no potential N-linked glycosylation sites. Based on other members of the family, HIN-1 is likely to exist as a disulfide-linked homodimer. The molecule is found in mammary and upper airway epithelium. Mature mouse HIN-1 shares 60% aa sequence identity with mature human HIN-1.
Applications
Suitable for use in direct ELISA and Western Blot. Other applications not tested.
Recommended Dilutions
Western Blot: 1ug/ml using lysates of rat lung tissue Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Phe22-Gly104 of mouse UGRP2 expressed in E. coli (Q920D7). Species Sequence Homology: 5% cross-reactivity with recombinant human HIN-1
Form
Supplied as a lyohilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse UGRP2. Species Crossreactivity: rat