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You are here:Home » Antibodies » Antibodies-Carbohydrates, Glycoproteins » Anti -Vascular Endothelial Statin (VE-Statin, EGF-Like Domain-containing Protein 7, EGFL7)

Anti -Vascular Endothelial Statin (VE-Statin, EGF-Like Domain-containing Protein 7, EGFL7)


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Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B
Mouse VE-Statin (vascular endothelial cell statin; also EGFL7) is a 41kD, secreted glycoprotein that belongs to an expanding family of EGF-like domain-containing proteins. It is an early marker of embryonic endothelial cells, and occurs in adult endothelium. Its secretion blocks smooth muscle migration. The mature molecule is 254 amino acids (aa) in length, and contains one N-terminal EMI domain (aa28-105), two EGF-like domains (aa 106-178), and a coiled-coil region (aa196-220). There is at least one isoform that shows a 13 aa deletion between aa236-248. Mature mouse VE-statin is 76% and 80% identical to dog and human VE-statin, respectively.
Catalog #V2111-05
ApplicationsSuitable for use in Western Blot and Direct ELISA. Other applications not tested.
Recommended DilutionsWestern Blot: 1-2ug/ml. The detection limit fot recombinant mouse VE-Statin is ~25ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity 5 to 50 fold.
Direct ELISA: 0.5-1ug/ml. The detection limit fot recombinant mouse VE-Statin is ~7ng/well.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No7H138
Concentration0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa21-275 from nouse VE-Statin expressed in E. Coli (Q9QXT5). Species Sequence Homology: human; 80%, canine; 76%
SpecificityRecognizes mouse VE-Statin. Shows no crossreactivity with recombinant human VE-Statin in Western Blot or Direct ELISA.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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