Zinc is an essential nutrient for all organisms because of the many important roles this metal plays. Movement of zinc into and out of cells and subcellular organelles is mediated by zinc transporter proteins. In many organisms, zinc uptake is mediated by members of the ZIP family of metal ion transporters. In mammals, the Zip1, Zip2, Zip3, Zip4, Zip5, LIV- 1 (Zip6), KE4 (Zip7), and BIGM103 (Zip8) proteins have been implicated in zinc uptake in a variety of cell and tissue types.
Zip1 (SLC39A1; IRT1; ZIRTL) was found in a human EST database by its homology with Arabidopsis Zip1 transporter. The 34kD human ZIP1, with 324aa residues is located at chromosome 1q21. The human and mouse ZIP1 proteins ZIP1 has a predicted topology of eight transmembrane domains (TMDs) and a leucine zipper DNA-binding motif. Overexpression of human or mouse ZIP1 has demonstrated the 65Zn uptake enhancing properties of Zip1.
Applications
Suitable for use in Western Blot, ELISA. Other applications not tested.
Recommended Dilution
Western Blot: 1-10ug/ml (Chemiluminescence) ELISA: 1:100,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to 16aa of mouse ZIP1 (Accession #Q9QZ03), N-terminal, Extracellular domain (KLH).
Form
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse ZIP1. Species Crossreactivity: human.
TDS
NCBI
UniProt
Tech Support
Customer Service
