Technical Data
0004-01K
4-1BB Ligand (4-1BBL)
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Technical Data |
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0004-01K |
4-1BB Ligand (4-1BBL) |
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Description: 4-1BB was originally described as a cDNA expressed by activated murine T cells and subsequently demonstrated to encode a member of the tumor necrosis factor receptor family of integral membrane proteins. Recently, a murine ligand for 4-1BB (mu4-1BB-L) was cloned and demonstrated to be a member of an emerging family of ligands with structural homology to tumor necrosis factor. Both monoclonal antibody to hu4-1BB and cells transfected with hu4-1BB-L induced a strong proliferative response in mitogen co-stimulated primary T cells. In contrast, ligation of 4-1BB on T cell clones enhanced activation-induced cell death when triggered by engagement of the TCR/CD3 complex. (1). To reject tumors, T cells must overcome poor tumor immunogenicity and an adverse tumor microenvironment. It has been shown that primary human T cells expressing CD80 and 4-1BBL vigorously respond to tumor cells lacking costimulatory ligands and provoke potent rejection of large, systemic tumors in immunodeficient mice. Applications: Suitable for use in Western Blot, Immunohistochemistry , Immunocytochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1:500-1:2000 Immunohistochemistry: 1:100-1:250 Immunocytochemistry: 1:100-1:250 Immunoprecipitation: 1:40 Optimal dilution determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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