Technical Data
4-1BB Ligand (4-1BBL)
4-1BB was originally described as a cDNA expressed by activated murine T cells and subsequently demonstrated to encode a member of the tumor necrosis factor receptor family of integral membrane proteins. Recently, a murine ligand for 4-1BB (mu4-1BB-L) was cloned
and demonstrated to be a member of an emerging family of ligands with structural homology to tumor necrosis factor. Both monoclonal antibody to hu4-1BB and cells transfected
with hu4-1BB-L induced a strong proliferative response in mitogen co-stimulated primary T cells. In contrast, ligation of 4-1BB on T cell clones enhanced activation-induced cell death when triggered by engagement of the TCR/CD3 complex. (1). To reject tumors, T cells must overcome poor tumor immunogenicity and an adverse tumor microenvironment. It has been shown that primary human T cells expressing CD80 and 4-1BBL vigorously respond to tumor cells lacking costimulatory ligands and provoke potent rejection of large, systemic tumors in immunodeficient mice.

Suitable for use in Western Blot, Immunohistochemistry , Immunocytochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:100-1:250
Immunocytochemistry: 1:100-1:250
Immunoprecipitation: 1:40
Optimal dilution determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
100ul-20°CBlue IceHumanRabbit
Not determined
A synthetic peptide corresponding to residues near the N-terminus of human 4-1BBL.
Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
Recognizes human 4-1BBL.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Alderson MR, et al. Eur J Immunol 24(9): 2219-27, 1994. 2. Steohan MT, et al. Nat Med 13(12): 1440-9, 2007