Technical Data
0004-01L
4-1BB Receptor (CD137, 4-1BB Ligand Receptor, TNFRSF9, CDw137, ILA, MGC2172, T-cell antigen 4-1BB homolog) (PE)
Description:
4-1BB is an inducible T cell surface protein belonging to the TNF receptor superfamily. It is alternatively known as TNFRSF9, CD137, and ILA. The 255aa human 4-1BB is a type I transmembrane protein having in its extracellular domain four of the cysteine-rich motifs that are characteristic of the TNF receptor superfamily. The 30kD glycoprotein exists both as a
monomer and as a dimer on T cells. The human and mouse proteins share 60% aa identity. 4-1BB is absent from naive T cells, but it is upregulated and continually expressed following T cell activation. The natural ligand, 4-1BBL, is a member of the TNF superfamily and is expressed on activated antigen presenting cells including dendritic cells, macrophages, and B cells. Cross- linking of 4-1BB by 4-1BBL or by agonistic antibodies transmits a potent co-stimulatory signal that enhances the effect of other activating signals such as PHA or anti-CD3 antibodies. 4-1BB signals through the TFAF2-NIK pathway resulting in activation of NF-kB and ultimately promoting proliferation and survival of T cells.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b9H3Purified
SizeStorageShippingSourceHost
100Tests-20CBlue IceHumanMouse
Concentration:
~0.05mg/ml
Immunogen:
Synthetic peptide corresponding to human 4-1BB/TNFRSF9.
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
Specificity:
Recognizes human 4-1BB/TNFRSF9 (PE).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.