		Technical Data
0004-01V	4-1BB Ligand (4-1BBL, 4-1BB-L, CD137L, CD157L, Homolog of Mouse 4-1BB-L, Homolog of Mouse 4-1BBL, ILA Ligand (TNF-related), Ly63L, Receptor 4-1BB Ligand, Tumor Necrosis Factor (Ligand) Superfamily Member 9)

Description:
Applications:
Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:10-1:50; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	10B723	Affinity Purified

Size		Storage	Shipping	Source	Host
250ug		4°C/-20°C (No Glycerol)	Blue Ice	Mouse	Rat

Concentration:

~1mg/ml

Immunogen:

CD137L-Fc Fusion Protein.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

Specificity:

Recognizes murine CD137L (4-1BBL).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Goodwin, R.G. et al. (1993) Molecular cloning of a ligand for the inducible T cell gene 4-1BB: a member of an emerging family of cytokines with homology to tumor necrosis factor. Eur. J. Immunol. 23: 2631-41.