Technical Data
4-1 BB Receptor, Recombinant, Human (4-1-BBR)
Molecular Biology Storage: -20CShipping: Blue Ice
Recombinant Human soluble 4-1BB Receptor also called Tumor necrosis factor receptor superfamily member 9 produced in E. coli is a single, non-glycosylated polypeptide chain containing 167 amino acids, having a molecular mass of 17.718kD and containing the cysteine rich TNFR-like extracellular domain of recombinant 4-1BB Receptor. The human 4-1BB Receptor is purified by proprietary chromatographic techniques.

Amino Acid Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Arg-Thr-Arg.

Dimers and Aggregates:
1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:
Recombinant 4-1BBr is fully biologically active when compared to standard. The activity was determined by the inhibition of 4-1BB ligand mediated stimulation of IL-8 production by human PBMC. Results: 90% inibition using 1g for both human 4-1BB ligand and human 4-1BB receptor.

0.1ng/ug (IEU/ug) of recombinant 4-1BBr.

It is recommended to reconstitute the lyophilized human 4-1BB Receptor in sterile 18M -cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Lyophilized recombinant human 4-1BB Receptor although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution human 1-4BB Receptor should be stored at 4C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Molecular Weight:
Source: E. coli
Purity: 98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Concentration: ~1mg/ml
Form: Supplied as a lyophilized powder without additives.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.