Technical Data
4-1 BB Receptor, Recombinant, Human, huIg Fusion Protein (CD137, 4-1BB Ligand Receptor, TNFRSF9, CDw137, ILA, MGC2172, T-cell antigen 4-1BB homolog) (Preservative Free)
Molecular Biology Storage: 4C Do Not FreezeShipping: Blue Ice
Human CD137 (4-1BB) is expressed on activated T cells within 24-48 hours of activation (3). CD137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily (1,2). CD137 appears to be important for T cell proliferation and survival (4).

Functional Application: CD137 Ig fusion protein blocks binding of anti-human CD137 to activated CEM human tumor cells and also binds to CD137 Ligand on Raji cells.

Human CD137(4-1BB) huIg Fusion Protein*

Molecular Structure: A soluble 55 kd fusion protein consisting of the extracellular (186aa) domain of human CD137 (4-1BB) fused to human IgG1 Fc (4).

Transfectant Cell Line: CHO

Suitable for use in ELISA and Flow cytometry. Other applications not tested.

Recommended Dilution
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: CHO cells
Purity: Purified by Protein A affinity chromatography.
Concentration: ~0.5mg/ml
Form: Supplied as a liquid in FBS, 50mM Sodium Phosphate pH 7.5, 100mM Potassium Chloride, 150mM NaCl.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
(1) M.R. Alderson, et al, (1994) Eur J Immunol 24: 2219-2227.
(2) H.J. Gruss, and S.K. Dower, (1995) Blood 85: 3378-3404.
(3) B.A. Garni-Wagner, et al, (1996) Cellular Immunol 169: 91-98.
(4) H. Schwarz, et al, (1996) Blood 87: 2839-2845

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.