Technical Data
4E Binding Protein 1,2,3 phosphorylated (Thr45) (4E-BP123, 4EBP1, 4EBP2, 4EBP3, 4E-BP1, Protein Synthesis Initiation Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP))
4E-BP1 is a eukaryotic translation initiation factor (eIF4E- binding protein), also known as PHAS-1. The binding of 4E-BP to eIF4E is regulated by phosphorylation and is reversible. Non-phosphorylated 4E-BP1 bind strongly to eIF4E; however, phosphorylated 4E-BP1 will not bind to eIF4E. Akt, TOR, MAP, S6, and Cdc2 kinases are able to inactivate 4E-BP1 by phosphorylation. 4E-BP1 undergoes phosphorylation which releases it from eIF4E, ultimately forming initiation complexes that aid in the upregulation of protein synthesis.

Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:5,000-10,000
Immunohistochemistry (paraffin): 1:50-100
Immunocytochemistry: 1:100-250
Immunoprecipitation: 1:10
Optimal dilutions to be determined by the researcher.

Positive Control:
Untreated and FBS-treated 293T cell lysate.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul-20CBlue IceHumanRabbit
Not determined
Phospho-specific peptide corresponding to residues surrounding Thr45 of human 4E-BP1/2/3.
Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
Recognizes 4E-BP1/2/3 phosphorylated at Thr45.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Pause, A., et al. (1994). Nature 371: 762-767. 2. Gingras, A.C., et al. (1998). Genes & Dev. 12: 502-513. 3. Iritani, B.M., et al. (1999). Proc. Natl. Acad. Sci. 96, 13180-13185. 4. Trumpp, A., et al. (2001). Nature 414, 768773.