Technical Data
0004-19J
4E Binding Protein 1,2,3 phosphorylated (Thr45) (4E-BP123, 4EBP1, 4EBP2, 4EBP3, 4E-BP1, Protein Synthesis Initiation Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP))
Description:
4E-BP1 is a eukaryotic translation initiation factor (eIF4E- binding protein), also known as PHAS-1. The binding of 4E-BP to eIF4E is regulated by phosphorylation and is reversible. Non-phosphorylated 4E-BP1 bind strongly to eIF4E; however, phosphorylated 4E-BP1 will not bind to eIF4E. Akt, TOR, MAP, S6, and Cdc2 kinases are able to inactivate 4E-BP1 by phosphorylation. 4E-BP1 undergoes phosphorylation which releases it from eIF4E, ultimately forming initiation complexes that aid in the upregulation of protein synthesis.

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:5,000-10,000
Immunohistochemistry (paraffin): 1:50-100
Immunocytochemistry: 1:100-250
Immunoprecipitation: 1:10
Optimal dilutions to be determined by the researcher.

Positive Control:
Untreated and FBS-treated 293T cell lysate.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG9i141Supernatant
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Phospho-specific peptide corresponding to residues surrounding Thr45 of human 4E-BP1/2/3.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
Specificity:
Recognizes 4E-BP1/2/3 phosphorylated at Thr45.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Pause, A., et al. (1994). Nature 371: 762-767. 2. Gingras, A.C., et al. (1998). Genes & Dev. 12: 502-513. 3. Iritani, B.M., et al. (1999). Proc. Natl. Acad. Sci. 96, 13180-13185. 4. Trumpp, A., et al. (2001). Nature 414, 768773.