Technical Data
4E Binding Protein 1, non-phosphorylated (Thr46) (Protein Synthesis Initiation Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP)
4E-BP1 (also known as PHAS-1) when bound to eIF4E, inhibits cap-dependent translation. Upon hyperphosphorylation of 4E-BP1 this interaction is disrupted and cap-dependent translation is activated (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4); while phosphorylation by FRAP/mTOR on Thr37 and Thr46 does not prevent the binding of 4E-BP-1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).

Suitable for use in Flow Cytometry, ELISA, Western Blot. Other applications not tested.

Recommended Dilution:
Western blot 1:1000
Flow Cytometry 1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul-20CBlue IceHumanRabbit
Not determined
Synthetic peptide (KLH-coupled) corresponding to residues surrounding Thr46 of human 4E-BP1.
Supplied as a liquid in 10mM sodium HEPES (pH 7.5), 150mM NaCl, 100ug/ml BSA, 50% glycerol and less than 0.02% sodium azide.
Detects endogenous levels of 4E-BP1 only when dephosphorylated at Thr46. Crossreacts with 4E-BP2 and 4E-BP3 dephosphorylated at equivalent sites.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
(1) Pause, A. et al. (1994) Nature 371, 762767. (2) Brunn, G.J. et al. (1997) Science 277, 99101. (3) Gingras, A.C. et al. (1998) Genes Dev. 12, 502513. (4) Fadden, P. et al. (1997) J. Biol. Chem. 272, 1024010247. (5) Gingras, A.C. et al. (1999) Genes Dev. 13, 14221437.