Technical Data
0014-02C
14-3-3, alpha, beta, NT (Protein 1054, Protein Kinase C Inhibitor Protein 1, brain protein 14-3-3, beta Isoform, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide, protein kinase C inhibitor protein-1)
Description:
The 14-3-3 proteins are a family of proteins involved in the regulation of apoptosis, mitogenic signaling and cell-cycle checkpoints (1). The 14-3-3 proteins are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins (2,3). Through binding Bad, 14-3-3 prevents apoptosis by sequestering Bad to the cytosol (3)

Applications:
Suitable for use in, Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:50
Western Blot: 1:1000
Immunohistochemistry: 1:250
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG6k4Supernatant
SizeStorageShippingSourceHost
100ul4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined.
Immunogen:
Synthetic peptide corresponding to residues near the N-terminus of human 14-3-3, beta, alpha.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide and 40% glycerol.
Specificity:
Recognizes human 14-3-3 beta/alpha. Species crossreactivity: mouse and rat. Species sequence homology: sheep, bovine
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Fu, H., et al. . Ann. Rev. Pharmacol. Toxicol. 40: 617-47 (2000). 2. Muslin, A.J. et al. Cell 84: 889–-97 (1996). 3. Zha, J. et al. Cell 87: 619-628 (1996).