Technical Data
0014-02C
14-3-3, alpha, beta, NT (Protein 1054, Protein Kinase C Inhibitor Protein 1, brain protein 14-3-3, beta Isoform, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide, protein kinase C inhibitor protein-1)
Description:
The 14-3-3 proteins are a family of proteins involved in the regulation of apoptosis, mitogenic signaling and cell-cycle checkpoints (1). The 14-3-3 proteins are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins (2,3). Through binding Bad, 14-3-3 prevents apoptosis by sequestering Bad to the cytosol (3)

Applications:
Suitable for use in, Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:50
Western Blot: 1:1000
Immunohistochemistry: 1:250
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG6k4Supernatant
SizeStorageShippingSourceHost
100ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined.
Immunogen:
Synthetic peptide corresponding to residues near the N-terminus of human 14-3-3, beta, alpha.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide and 40% glycerol.
Specificity:
Recognizes human 14-3-3 beta/alpha. Species crossreactivity: mouse and rat. Species sequence homology: sheep, bovine
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Fu, H., et al. . Ann. Rev. Pharmacol. Toxicol. 40: 617-47 (2000). 2. Muslin, A.J. et al. Cell 84: 889-97 (1996). 3. Zha, J. et al. Cell 87: 619-628 (1996).