Technical Data
0014-02S
14-3-3 beta (Tyrosine 3-monooxygenase/tryptopan 5-monooxygenase Activation Protein beta Polypeptide, YWHAB)
Description:
14-3-3, a family of acidic and soluble proteins, highly conserved in aa sequences from yeast to mammals, is expressed in all eukaryotic cells. Seven isoforms(beta, gamma, epsilon, eta, zeta, sigma and tau/phi) encoded by seven distinct genes are identified in mammals and forms homo- and hetero- dimeric cup-shaped structures. As 14-3-3 is interacted with more than 100 binding partners, it regulates key proteins involved in various biological processes such as signal trans-duction, cell cycle, transcriptional control, cell proliferation, apoptosis, and ion channel physiology. Most 14-3-3 requires phosphorylation of serine or threonine residues in the target sequence. This protein is abundantly expressed in the brain and has been detected in the cerebrospinal fluid of patients with different neurological disorders.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:2000
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa cells

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Recombinant corresponding to human 14-3-3 beta expressed in E.coli.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in HEPES, 0.15M sodium chloride, 0.01% BSA, 0.03% sodium azide, 50% glycerol.
Specificity:
Recognizes human 14-3-3 beta. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Tzivion, G. et al. (2001) Oncogene, 20, 6331-6338. 2. Tzivion, G. and Avruch, J. (2002) J.Biol.Chem. 277, 3061-3064. 3. Berg, D. et al. (2003) Nat. Rev. Neurosci. 4(9), 752-762.