Technical Data
029061
ATP6V1C1 (V-type Proton ATPase Subunit C 1, V-ATPase Subunit C 1, Vacuolar Proton Pump Subunit C 1, ATP6C, ATP6D, VATC) (HRP)
Description:
This protein is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
50ug-20CBlue IceRatRabbit
Concentration:
As reported
Immunogen:
Recombinant corresponding to human V-type proton ATPase subunit G 1.
Purity:
Purified by ammonium sulfate precipitation.
Form
Supplied as a liquid in 0.01M PBS, PH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with horseradish peroxidase (HRP).
Specificity:
Recognizes rat ATP6V1C1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. "Identification of genes expressed in human CD34(+) hematopoietic stem/progenitor cells by expressed sequence tags and efficient full-length cDNA cloning."Mao M., Fu G., Wu J.-S., Zhang Q.-H., Zhou J., Kan L.-X., Huang Q.-H., He K.-L., Gu B.-W., Han Z.-G., Shen Y., Gu J., Yu Y.-P., Xu S.-H., Wang Y.-X., Chen S.-J., Chen Z.Proc. Natl. Acad. Sci. U.S.A. 95:8175-8180 (1998). 2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."The MGC Project TeamGenome Res. 14:2121-2127 (2004). 3. "Molecular cloning and characterization of novel tissue-specific isoforms of the human vacuolar H(+)-ATPase C, G and d subunits, and their evaluation in autosomal recessive distal renal tubular acidosis."Smith A.N., Borthwick K.J., Karet F.E.Gene 297:169-177 (2002).