Technical Data
ATP6V1E1 (V-type Proton ATPase Subunit E 1, V-ATPase Subunit E 1, V-ATPase 31kD Subunit, p31, Vacuolar Proton Pump Subunit E 1, ATP6E, ATP6E2) (FITC)
Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.

Suitable for use in FLISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
50ug-20CBlue IceHumanRabbit
As reported
Recombinant corresponding to human ATP6V1E1.
Purified by ammonium sulfate precipitation.
Supplied as a liquid in 0.01M PBS, PH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with fluorescein isothiocyanate (FITC).
Recognizes human ATP6V1E1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. "Immunologic evidence that vacuolar H+ ATPases with heterogeneous forms of Mr = 31,000 subunit have different membrane distributions in mammalian kidney."Hemken P., Guo X.-L., Wang Z.-Q., Zhang K., Gluck S.J. Biol. Chem. 267:9948-9957 (1992). 2. "The E subunit of vacuolar H(+)-ATPase localizes close to the centromere on human chromosome 22."Baud V., Mears A., Lamour V., Scamps C., McDermid A., Lipinski M.Hum. Mol. Genet. 3:335-339 (1994). 3. "Cloning and tissue distribution of subunits C, D, and E of the human vacuolar H(+)-ATPase." van Hille B., Vanek M., Richener H., Green J.R., Bilbe G.Biochem. Biophys. Res. Commun. 197:15-21 (1993).