Technical Data
029067
ATP6V1E1 (V-type Proton ATPase Subunit E 1, V-ATPase Subunit E 1, V-ATPase 31kD Subunit, p31, Vacuolar Proton Pump Subunit E 1, ATP6E, ATP6E2) (Biotin)
Description:
Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Recombinant corresponding to human ATP6V1E1.
Purity:
Purified by ammonium sulfate precipitation.
Form
Supplied as a liquid in 0.01M PBS, PH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with Biotin.
Specificity:
Recognizes human ATP6V1E1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. "Immunologic evidence that vacuolar H+ ATPases with heterogeneous forms of Mr = 31,000 subunit have different membrane distributions in mammalian kidney."Hemken P., Guo X.-L., Wang Z.-Q., Zhang K., Gluck S.J. Biol. Chem. 267:9948-9957 (1992). 2. "The E subunit of vacuolar H(+)-ATPase localizes close to the centromere on human chromosome 22."Baud V., Mears A., Lamour V., Scamps C., McDermid A., Lipinski M.Hum. Mol. Genet. 3:335-339 (1994). 3. "Cloning and tissue distribution of subunits C, D, and E of the human vacuolar H(+)-ATPase." van Hille B., Vanek M., Richener H., Green J.R., Bilbe G.Biochem. Biophys. Res. Commun. 197:15-21 (1993).