Technical Data
ACTN3, NT (Alpha-actinin-3, Alpha-actinin Skeletal Muscle Isoform 3, F-actin Cross-linking Protein)
The alpha-actinins are a multigene family of four actin-binding proteins related to dystrophin. The two skeletal muscle isoforms of alpha-actinin (ACTN2 and ACTN3) are major structural components of the Z-line involved in anchoring the actin-containing thin filaments. In humans, ACTN2 is expressed in all muscle fibres, while ACTN3 expression is restricted to a subset of type 2 fibers. Absence of alpha-actinin-3 is not associated with an obvious disease phenotype, raising the possibility that ACTN3 is functionally redundant in humans, and that alpha-actinin-2 is able to compensate for alpha-actinin-3 deficiency. Alpha-Actinin-3 is specifically expressed in fast-twitch myofibers responsible for generating force at high velocity. The absence of a disease phenotype secondary to alpha-actinin-3 deficiency is likely due to compensation by the homologous protein, alpha-actinin-2. However, the high degree of evolutionary conservation of ACTN3 suggests function(s) independent of ACTN2. Highly significant associations between ACTN3 genotype and athletic performance have been demonstrated in both male and female athletes.

Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:10,000
Immunocytochemistry: 1:100-1:250
Immunohistochemistry (paraffin): 1:100-1:250
Flow Cytometry: 1:200
Optimal dilutions to be determined by the researcher.

Positive Control:
Human skeletal muscle tissue lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceHumanRabbit
Not determined
Synthetic peptide corresponding to human ACTN3 at N-terminal.
Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
Recognizes human ACTN3. Species Crossreactivity: Mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Mills, M., et al. (2001). Hum Mol Genet. 10(13):1335-1346. 2. Yang, N., et al. (2003). Am J Hum Genet. 73(3):627-631.