		Technical Data
029417	Angiotensinogen (Serpin A8, AGT, SERPINA8)

Description:
Angiotensinogen is a precursor of angiotensin-1,2, and 3; as a member of the serpin family, it is expressed by the liver and secreted in plasma. In response to lowered blood pressure, renin cleaves angiotensinogen and produce angiotensin-1. Angiotensin-converting enzyme (ACE) further removes a dipeptide to produce the physiologically active peptide angiotensin-2. Angiotensin-2 is involved in regulating mineral balance and volume of body fluids, while angiotensin-3 stimulates aldosterone release. Defects in angiotensinogen have been linked to renal tubular dygenesis (RTD) and susceptibility to essential hypertension.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-10,000
Immunocytochemistry: 1:100-1:250
Immunoprecipitation: 1:10-1:100
Optimal dilutions to be determined by the researcher.

Positive Control:
Fetal artery, fetal heart, fetal liver, HepG2, 293T, and human plasma lysates

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG	13A156	Supernatant

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

Not determined

Immunogen:

Synthetic peptide corresponding to human Angiotensinogen.

Purity:

Supernatant

Form

Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.

Specificity:

Recognizes human Angiotensinogen. Species Crossreactivity: Mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Tainmoto K., et al. (1994). JBC. 269(50):31334-31337. 2. Kilian P., et al. (2008). Endocrinology. 149(6):2923-2933. 3. The UniProt Consortium. (2010). The Universal Protein Resource (UniProt) Nucleic Acids Res. 36:D190-D195.