Technical Data
029417
Angiotensinogen (Serpin A8, AGT, SERPINA8)
Description:
Angiotensinogen is a precursor of angiotensin-1,2, and 3; as a member of the serpin family, it is expressed by the liver and secreted in plasma. In response to lowered blood pressure, renin cleaves angiotensinogen and produce angiotensin-1. Angiotensin-converting enzyme (ACE) further removes a dipeptide to produce the physiologically active peptide angiotensin-2. Angiotensin-2 is involved in regulating mineral balance and volume of body fluids, while angiotensin-3 stimulates aldosterone release. Defects in angiotensinogen have been linked to renal tubular dygenesis (RTD) and susceptibility to essential hypertension.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-10,000
Immunocytochemistry: 1:100-1:250
Immunoprecipitation: 1:10-1:100
Optimal dilutions to be determined by the researcher.

Positive Control:
Fetal artery, fetal heart, fetal liver, HepG2, 293T, and human plasma lysates

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG13A156Supernatant
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to human Angiotensinogen.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
Specificity:
Recognizes human Angiotensinogen. Species Crossreactivity: Mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Tainmoto K., et al. (1994). JBC. 269(50):31334-31337. 2. Kilian P., et al. (2008). Endocrinology. 149(6):2923-2933. 3. The UniProt Consortium. (2010). The Universal Protein Resource (UniProt) Nucleic Acids Res. 36:D190-D195.