		Technical Data
029420	ATG12 (Ubiquitin-like Protein ATG12, Autophagy-related Protein 12, APG12-like, APG12, APG12L)

Description:
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. Atg12 is the human homolog of a yeast protein involved in autophagy. It is essential for Atg12 conjugation, and the Atg12-Atg5 conjugate is necessary to form autophagic vesicles. The E3-like activity of the Atg12-Atg5 conjugate potentiates the formation of Atg8-PE; making the autophagic pathway analogous to ubiquitin-like pathways where an E3 conjugation ligase is required.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-10,000
Immunohistochemistry (Paraffin): 1:100-1:250
Immunocytochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.

Positive Control:
Fetal kidney tissue lysate, HepG2 and Raji cell lysates

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG	13A158	Supernatant

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

Not determined

Immunogen:

Synthetic peptide corresponding to human ATG12 at C-terminal.

Purity:

Supernatant

Form

Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.

Specificity:

Recognizes human ATG12. Species sequence homology: Mouse and rat (100%).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Entrez Gene: gene-centered information at NCBI. (2005). Nucleic Acids Res. 33:D54- D58. 2. Phillips, A.R., et al. (2008). Genetics. 178(3):1339-1353. 3. Williams, R.A., et al. (2009). Autophagy. 5(2):159-172. 1. 4. Huang, J., et al. (2009) PNAS. 106(15):6226- 6231.