Technical Data
ATG12 (Ubiquitin-like Protein ATG12, Autophagy-related Protein 12, APG12-like, APG12, APG12L)
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. Atg12 is the human homolog of a yeast protein involved in autophagy. It is essential for Atg12 conjugation, and the Atg12-Atg5 conjugate is necessary to form autophagic vesicles. The E3-like activity of the Atg12-Atg5 conjugate potentiates the formation of Atg8-PE; making the autophagic pathway analogous to ubiquitin-like pathways where an E3 conjugation ligase is required.

Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-10,000
Immunohistochemistry (Paraffin): 1:100-1:250
Immunocytochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.

Positive Control:
Fetal kidney tissue lysate, HepG2 and Raji cell lysates

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceHumanRabbit
Not determined
Synthetic peptide corresponding to human ATG12 at C-terminal.
Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
Recognizes human ATG12. Species sequence homology: Mouse and rat (100%).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Entrez Gene: gene-centered information at NCBI. (2005). Nucleic Acids Res. 33:D54- D58. 2. Phillips, A.R., et al. (2008). Genetics. 178(3):1339-1353. 3. Williams, R.A., et al. (2009). Autophagy. 5(2):159-172. 1. 4. Huang, J., et al. (2009) PNAS. 106(15):6226- 6231.