Technical Data
ATG5 (Autophagy Protein 5, APG5-like, Apoptosis-specific Protein, APG5L, ASP)
Atg5 (Autophagy protein 5) is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. Atg5 may also play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.

Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:100-10,000
Immunocytochemistry: 1:100-1:250
Immunohistochemistry (paraffin): 1:100-1:250
Immunoprecipitation: 1:10
Optimal dilutions to be determined by the researcher.

Positive Control:
Raji, HeLa, HT-1080, fetal kidney, C6, Raw264.7, PC-12, and NIH/3T3 cell lysates

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceHumanRabbit
Not determined
Synthetic peptide corresponding to human ATG5.
Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
Recognizes human ATG5. Species Crossreactivity: Mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Entrez Gene: gene-centered information at NCBI. (2005). Nucleic Acids Res. 33:D54-D58. 2. Phillips, A.R., et al. (2008). Genetics. 178(3):1339-1353. 3. Williams, R.A., et al. (2009). Autophagy. 5(2):159-172. 1. 4. Huang, J., et al. (2009) PNAS. 106(15):6226-6231.