		Technical Data
029493	HDAC2, CT (Histone Deacetylase 2, HD2)

Description:
Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly conserved from yeast to human.

Applications:
Suitable for use in Immunoprecipitation and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Positive Control:
Normal mouse IgG and primers specific for human von Willebrand factor.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	13A192	Affinity Purified

Size		Storage	Shipping	Source	Host
25 Tests		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Synthetic peptide CEKTDTKGTKSEQLSNP corresponding to human HDAC2 at C-terminal (KLH).

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.

Specificity:

Recognizes human HDAC2. Species Crossreactivity: Bovine, mouse and hamster. Species sequence homology: rat

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.