		Technical Data
029610	SIRT5 (NAD-dependent Deacetylase Sirtuin 5, SIR2-like Protein 5, SIR2L5)

Description:
Sirtuin (SIRT5) is known as a nicotinamide adenine dinucleotide- dependent deacetylase that is a member of the silent information regulator 2 (Sir2) family of proteins that is important in aging, metabolism and disease. SIRT5 is expressed in a variety of tissues. Two different isoform have been described. Isoform 1 is reported to be express in the mitochondria and cytoplasm while isoform 2 appears to be localized to the mitochondria only. SIRT5 has also been linked to many primate-specific functions. SIRT5 has recently been implicated as having a role in adaptation to fasting, caloric restriction and high protein diets.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5ug/ml
Immunohistochemistry: 1:500
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa cell lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~1mg/ml

Immunogen:

Synthetic linear peptide corresponding to human SIRT5 (KLH).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

Specificity:

Recognizes human SIRT5. Species Crossreactivity: rat. Species sequence homology: chimpanzee and rhesus macaque (100%).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Matsushita, N., et al. (2011). Genes Cells. 16(2):190-202. 2. Nakagawa, T. and Guarente, L. (2009). Aging (Albany NY). 1(6):578-581. 3. Mahlknecht, U., et al. (2006). Cytogenet Genome Res. 112(3-4):208-212.