Technical Data
029627
TRAF2 (TNF Receptor-associated Factor 2, E3 Ubiquitin-protein Ligase TRAF2, Tumor Necrosis Factor Type 2 Receptor-associated Protein 3, TRAP3)
Description:
TNF receptor associated factor 2 (TRAF2), an adaptor protein and ubiquitin ligase, is a member of the TRAF protein family that is involved in mediating signals from the tumor necrosis factor receptors (TNFRs). This protein directly interacts with TNF receptors, and forms a heterodimeric complex with TRAF1. The protein complex formed by TRAF2 and TRAF1 interacts with the inhibitor-of-apoptosis proteins and functions as a mediator of the anti-apoptotic signals from TNF receptors.

Applications:
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1ug/ml
Immunocytochemistry: 1:500
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa cell lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic linear peptide corresponding to human TRAF2 at the zinc finger (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes human TRAF2. Species Crossreactivity: Mouse. Species sequence homology: rhesus macaque, Xenopus (92%), rat (73%).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kim, J.Y., et al. (2011). J Cell Sci. 124(4):647-656. 2. Wu, C.J., et al. (2005). EMBO J. 24(10):1886-1898. 3. Grech, A.R., et al. (2004). Immunity. 21(5): 629-642. 4. Yoneda, T., et al. (2000). J Biol Chem. 275(15):1114-11120.