Technical Data
030760
ALB (ALB, Serum albumin)
Description:
Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:100~1:500
Optimal dilution to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a500CT2.1.4Affinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanMouse
Concentration:
As Reported
Immunogen:
KLH-conjugated synthetic peptide mapping to a fragment of amino acids within aa540-569 of human Albumin, UniProt Accession #P02768. Species Sequence Homology: monkey
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide
Specificity:
Recognizes human Albumin
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Schelleman, H., et al. Br J Clin Pharmacol 70(3):393-399(2010). 2. Minchiotti, L., et al. Hum. Mutat. 29(8):1007-1016(2008). 3. Rikova, K., et al. Cell 131(6):1190-1203(2007). 4. Sugio, S., et al. Protein Eng. 12(6):439-446(1999). 5. Sakamoto, Y., et al. Biochim. Biophys. Acta 1252(2):209-216(1995)