Technical Data
031571
ACTH, phosphorylated (S168) (POMC, Pro-opiomelanocortin, Corticotropin-lipotropin, NPP, Melanotropin gamma, Gamma-MSH, Potential peptide, Corticotropin, Adrenocorticotropic hormone, Melanotropin alpha, Alpha-MSH, Corticotropin-like intermediary peptide, L
Description:
This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq].

Applications:
Suitable for use in Dot Blot, ELISA

Recommended Dilution:
ELISA: 1:1,000
Dot blot 1:100-500

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Phospho-ACTH-pS168 Antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected surrounding S168 of human ACTH.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Canzian, F., et al. Hum. Mol. Genet. 19(19):3873-3884(2010)
Hoftberger, R., et al. Endocrinology 151(10):4801-4810(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Mastrofrancesco, A., et al. J. Immunol. 185(3):1903-1911(2010)
Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010)