Technical Data
031817
ALPP, NT (ALPP, PLAP, Alkaline phosphatase, placental type, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1)
Description:
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1:1000
Immunohistochemistry: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 56-83 in the N-terminal region of human ALPP, UniProt Accession #P05187.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Recognizes human ALPP.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Stec, B., et al. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 66 (PT 8), 866-870 (2010) : Wang, F., et al. Am. J. Surg. Pathol. 33(10):1529-1539(2009) Estrada, K., et al. Hum. Mol. Genet. 18(18):3516-3524(2009) Zhu, J.F., et al. Zhonghua Wai Ke Za Zhi 47(5):381-384(2009) Roberson, J.R., et al. Pediatr Blood Cancer 51(6):840-842(2008)