Technical Data
032272-Biotin
ATP1B3, CT (ATP1B3, Sodium/potassium-transporting ATPase subunit beta-3, Sodium/potassium-dependent ATPase subunit beta-3, CD298) (Biotin)
Description:
The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 3 subunit. This gene encodes a beta 3 subunit. A pseudogene exists for this gene, and it is located on chromosome 2. [provided by RefSeq].

Applications:
Suitable for use in Western Blot, Immunohistochemistry, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
ATP1B3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 248-278 amino acids from the C-terminal region of human ATP1B3.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Biotin.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Floyd, R.V., et al. Reprod Sci 17(4):366-376(2010)
Aughey, R.J., et al. J. Appl. Physiol. 103(1):39-47(2007)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Chiampanichayakul, S., et al. Tissue Antigens 68(6):509-517(2006)
Chi, A., et al. J. Proteome Res. 5(11):3135-3144(2006)