Technical Data
034542-APC
DDX11, ID (DDX11, CHL1, CHLR1, KRG2, Probable ATP-dependent RNA helicase DDX11, CHL1-related protein 1, DEAD/H box protein 11, Keratinocyte growth factor-regulated gene 2 protein) (APC)
Description:
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX11 encodes a DEAD box protein, which is an enzyme that possesses both ATPase and DNA helicase activities. DDX11 is a homolog of the yeast CHL1 gene, and may function to maintain chromosome transmission fidelity and genome stability.

Applications:
Suitable for use in Western Blot, Flow Cytometry, FLISA

Recommended Dilution:
FLISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul4C Do Not FreezeBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
DDX11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 663-691 amino acids from the Central region of human DDX11.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Allophycocyanin (APC).
Specificity:
Human, mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Leman, A.R., et al. J. Cell. Sci. 123 (PT 5), 660-670 (2010) :
Farina, A., et al. J. Biol. Chem. 283(30):20925-20936(2008)
Parish, J.L., et al. Mol. Cell 24(6):867-876(2006)
Parish, J.L., et al. J. Cell. Sci. 119 (PT 23), 4857-4865 (2006) :
Vasa-Nicotera, M., et al. Am. J. Hum. Genet. 76(1):147-151(2005)