Technical Data
035573
FBXW5, ID (FBXW5, FBW5, F-box/WD repeat-containing protein 5, F-box and WD-40 domain-containing protein 5)
Description:
This gene encodes a member of the F-box protein family, members of which are characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into three classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene contains WD-40 domains, in addition to an F-box motif, so it belongs to the Fbw class. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene, however, they were found to be nonsense-mediated mRNA decay (NMD) candidates, hence not represented.

Applications:
Suitable for use in Western Blot, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
FBXW5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 324-350 amino acids from the Central region of human FBXW5.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Minoda, Y., et al. Biochem. Biophys. Res. Commun. 381(3):412-417(2009)
Hu, J., et al. Genes Dev. 22(7):866-871(2008)
Wan, D., et al. Proc. Natl. Acad. Sci. U.S.A. 101(44):15724-15729(2004)
Humphray, S.J., et al. Nature 429(6990):369-374(2004)