Technical Data
038733
MYL12B, NT (MYL12B, MRLC2, MYLC2B, Myosin regulatory light chain 12B, MLC-2A, Myosin regulatory light chain 2-B, smooth muscle isoform, Myosin regulatory light chain 20kD, Myosin regulatory light chain MRLC2, SHUJUN-1)
Description:
The activity of nonmuscle myosin II (see MYH9; MIM 160775) is regulated by phosphorylation of a regulatory light chain, such as MRLC2. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments (Iwasaki et al., 2001 [PubMed 11942626]).

Applications:
Suitable for use in Western Blot, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
MYL12B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human MYL12B.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Martins-de-Souza, D., et al. Eur Arch Psychiatry Clin Neurosci 259(3):151-163(2009)
Umeda, D., et al. Biochem. Biophys. Res. Commun. 333(2):628-635(2005)
Li, T.B., et al. Acta Biochim. Biophys. Sin. (Shanghai) 36(6):412-418(2004)
Webb, R.C. Adv Physiol Educ 27 (1-4), 201-206 (2003) :
Iwasaki, T., et al. Cell Struct. Funct. 26(6):677-683(2001)