Technical Data
039805
PDE6A, ID (PDE6A, PDEA, Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha, PDE V-B1)
Description:
This gene encodes the cyclic-GMP (cGMP)-specific phosphodiesterase 6A alpha subunit, expressed in cells of the retinal rod outer segment. The phosphodiesterase 6 holoenzyme is a heterotrimer composed of an alpha, beta, and two gamma subunits. cGMP is an important regulator of rod cell membrane current, and its dynamic concentration is established by phosphodiesterase 6A cGMP hydrolysis and guanylate cyclase cGMP synthesis. The protein is a subunit of a key phototransduction enzyme and participates in processes of transmission and amplification of the visual signal. Mutations in this gene have been identified as one cause of autosomal recessive retinitis pigmentosa.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
~0.1mg/ml
Immunogen:
PDE6A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 308-336 amino acids from the Central region of human PDE6A. Species sequence homology: mouse and bovine.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Recognizes human PDE6A.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Clark, G.R., et al. Ophthalmology 117(11):2169-2177(2010) 2.Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) 3. Perlis, R.H., et al. Biol. Psychiatry 67(11):1110-1113(2010) 4. Bazhin, A.V., et al. Cell. Mol. Life Sci. 67(5):817-828(2010) 5. Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)