Technical Data
039963
PHLPP2, CT (PHLPP2, KIAA0931, PHLPPL, PH domain leucine-rich repeat-containing protein phosphatase 2, PH domain leucine-rich repeat-containing protein phosphatase-like)
Description:
PHLPP2 is a protein phosphatase that specifically mediates dephosphorylation of 'Ser-473' of AKT1. This protein regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and decrease cell proliferation. It also controls the phosphorylation of AKT3.

Applications:
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:50-100
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
PHLPP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1301-1331 amino acids from the C-terminal region of human PHLPP2.
Purity:
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Brognard,J., Mol. Cell 25 (6), 917-931 (2007)