Technical Data
040556
PSMB10, CT (PSMB10, LMP10, MECL1, Proteasome subunit beta type-10, Low molecular mass protein 10, Macropain subunit MECl-1, Multicatalytic endopeptidase complex subunit MECl-1, Proteasome MECl-1, Proteasome subunit beta-2i)
Description:
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome.

Applications:
Suitable for use in Western Blot and ELISA. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As Reported
Immunogen:
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 214-242 in the C-terminal region of human PSMB10, UniProt Accession #P40306.
Purity:
Purified by Protein A affinity chromatography
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide
Specificity:
Recognizes human PSMB10.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bailey, S.D., et al. Diabetes Care (2010). 2. Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). 3. Moschonas, A., et al. Mol. Cell. Biol. 28(20):6208-6222(2008). 4. Liu, Y., et al. DNA Seq. 18(4):257-264(2007). 5. Listovsky, T., et al. EMBO J. 23(7):1619-1626(2004)