Technical Data
044625
Cardif (CARD Adapter Inducing Interferon-beta, IPS-1, MAVS, Mitochondrial Antiviral Signaling Protein, VISA, Virus-induced Signaling Adapter, Interferon-beta Promoter Stimulator Protein 1)
Description:
RIG-I (retinoic acid-inducible gene I; Ddx58) and MDA5 (melanoma differentiation-associated gene 5, also known as Ifih1 or Helicard) are proteins that sense viral replication intermediates, such as double-stranded RNA and triggers the host antiviral programs. These molecules signal the downstream activation of NF-kappaB and IFN regulatory factor (IRF) -3, which coordinately regulate the expression of type-I interferons. Cardif (also called VISA/IPS-1/MAVS) is a new CARD (caspase activation and recruitment domain)-containing adaptor protein that interacts with the CARD domain of RIG-I and MDA5, leading to the activation of NF-kappaB and IRF3. Cardif is located to the mitochondrial outer membrane. Removal of the mitochondrial-targeting domain of cardif abolishes its ability to induce IFNs. Cardif is cleaved and inactivated by NS3-4A, a serine protease from hepatitis C virus known to block interferon-beta production.

Applications:
Suitable for use in Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot

Recommended Dilution:
Optimal dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2bAdri-1Highly Purified
SizeStorageShippingSourceHost
100ug4C/-20CBlue IceHumanMouse
Concentration:
1mg/ml
Immunogen:
Recombinant human Cardif (aa 160-450).
Purity:
>95% (SDS-PAGE)
Form
Supplied as a liquid in PBS, 10% glycerol, 0.02% sodium azide.
Specificity:
Recognizes human Cardif.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Product Reference:
TRADD Protein Is an Essential Component of the RIG-like Helicase Antiviral Pathway: M.C. Michallet, et al.; Immunity 28, 651 (2008)
Cleavage of mitochondrial antiviral signaling protein in the liver of patients with chronic hepatitis C correlates with a reduced activation of the endogenous interferon system: P. Bellecave, et al.; Hepatology 51, 1127 (2010)