Technical Data
IL-33, Recombinant, Human (His) (Interleukin-33, IL-1F11, NF-HEV)
Molecular Biology Storage: -20CShipping: Blue Ice
Interleukin-33 (IL-33; HF-NEV; IL-1F11), a member of the IL-1 family of cytokines, is expressed by many cell types following pro-inflammatory stimulation and is thought to be released on cell lysis. The 30kD human IL33 is converted by CASP1 to a 18kD protein. IL33 binds to and signals through ST2 (IL1R1) and its stimulation recruits MYD88, IRAK, IRAK4, and TRAF6, followed by phosphorylation of ERK1 (MAPK3)/ERK2 (MAPK1), p38 (MAPK14), and JNK. The ability of IL-33 to target numerous immune cell types, like Th2-like cells, mast cells, and B1 cells, and to induce cytokine and chemokine production underlines its potential in influencing the outcome of a wide range of diseases, such as arthritis, asthma, atopic allergy & anaphylaxis, cardiovascular disease/atherosclerosis, nervous system diseases, and sepsis.

Recombinant protein corresponding to aa112-270, fused at the C-Terminus with a His-Tag

Biological Activity:
Activates human ST2-dependent NF-kB pathway.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~18kD (SDS-PAGE)
Source: E. coli
Purity: ~90% (SDS-PAGE). Endotoxin: 1EU/ug (LAL)
Concentration: ~1mg/ml
Form: Supplied as a liquid in 55mM Tris-Cl, pH 8.2, 150mM sodium chloride.
Specificity: Binds to human ST2.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. IL-33 can promote survival, adhesion and cytokine production in human mast cells: M. Iikura, et. al; Lab. Invest. 87, 971 (2007). 2. Interleukin-33 enhances adhesion, CD11b expression and survival in human eosinophils: M. Suzukawa, 3. An IL-1 cytokine member, IL-33, induces human basophil activation via its ST2 receptor: M. Suzukawa, et al.; J. Immunol. 181, 5981 (2008)

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.