Technical Data
045088
NLRP3/NALP3 (NACHT-, LRR- and PYD-containing Protein 3, NLRP3, Cryopyrin, Cold Autoinflammatory Syndrome 1 Protein)
Description:
The inflammasome is a multiprotein complex that mediates the activation of caspase-1, which promotes, amongst others, the secretion of the proinflammatory cytokines interleukin (IL)-1beta and IL-18. Members of the Nod-like receptor family, including NLRP3/NALP3, are critical components of the inflammasome that link danger-signals to caspase-1 activation. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1), Muckle-Wells syndrome (MWS) and of chronic infantile neurologic cutaneous and articular syndrome (CINCA).

Applications:
Suitable for use in Western Blot

Recommended Dilution:
Optimal dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2bCryo-2Highly Purified
SizeStorageShippingSourceHost
100ug4C/-20CBlue IceMouseMouse
Concentration:
1mg/ml
Immunogen:
Recombinant mouse NLRP3/NALP3 (pyrin domain/aa 1-93).
Purity:
>95% (SDS-PAGE)
Form
Supplied as a liquid in PBS, 10% glycerol, 0.02% sodium azide.
Specificity:
Recognizes mouse and human NLRP3/NALP3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Product Reference:
Thioredoxin-interacting protein links oxidative stress to inflammasome activation: R. Zhou, et al.; Nature Immunology 11, 136 (2010)
The NLRP3 inflammasome promotes renal inflammation and contributes to CKD: A. Vilaysane, et al.; J. Am. Soc. Nephrol. 21, 1732 (2010)
A role for mitochondria in NLRP3 inflammasome activation: R. Zhou, et al.; Nature 469, 221 (2011)
Differential expression of NLRP3 among hematopoietic cells: G. Guarda, et al.; J. Immunol. 186, 2529 (2011)
Mechanism of Impaired NLRP3 Inflammasome Priming by Monophosphoryl Lipid A: C.A. Embry, et al.; Sci. Signal. 4, ra28 (2011)