Technical Data
045235
Resistin (FIZZ3, Found in Inflammatory Zone 3, ADSF, Adipose Tissue-Specific Secretory Factor)
Description:
The adipocytokine resistin which belongs to a family of cysteine-rich C-terminal proteins known as resistin-like molecules (RELM; RELMalpha/FIZZ 1 and RELMbeta/FIZZ 2) of FIZZ (found in inflammatory zone) are thought to be involved in inflammatory processes. Previous studies in mice showed that resistin impairs glucose tolerance and insulin action. In addition, resistin also inhibits adipogenesis in murine 3T3-L1 cells. Therefore resistin has also been proposed as an adipocyte-secreted factor thought to link obesity and T2DM.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA (Direct and Indirect): 1:5000-1:10,000
Western Blot: 1:2000-1:10,000 (ECL). Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20, 5% skim milk; suggested incubation time is 1 hour at RT
Optimal dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Recombinant protein corresponding to human resistin.
Purity:
Purified by Protein A affinity chromatography
Form
Supplied as a liquid in PBS, pH 7.4. No preservative added.
Specificity:
Recognizes human resistin at ~10kD. Weakly crossreacts with mouse resistin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.