Technical Data
045648
Prostate Specific Membrane Antigen (PSMA, FOLH1, FGCP, FGCP, GCP2, NAALAD1, NAALAdase, Glutamate carboxypeptidase 2, Pteroylpoly-gamma-glutamate carboxypeptidase)
Description:
PSMA (Prostate-specific membrane antigen, Glutamate carboxypeptidase 2) is a membrane peptidase expressed in the prostate, central and peripheral nervous system, kidney, and small intestine (1). PSMA has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. It is required in the intestine for the uptake of folate, and in the brain it modulates excitatory neurotransmission through the hydrolysis of the neuropeptide N-acetylaspartylglutamate, thereby releasing glutamate (2). In the central nervous system, it is responsible for the cleavage of N-acetyl- L-aspartyl-L-glutamate yielding free glutamate in the synaptic cleft, and is implicated in various pathologic conditions associated with glutamate excitotoxicity (1). PSMA also has abundant and restricted expression on the surface of prostate carcinomas and the neovasculature of most other solid tumors, making it a target for cancer immunotherapy (3).

Applications:
Suitable for use in Western Blot, Immunocytochemistry, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:50,000-1:200,000
Immunocytochemistry: 1:100-1:250
Immunoprecipitation: 1:10-1:100
Immunohistochemistry: 1:250-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabEPR6253Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Recombinant protein fragment corresponding to aa1-106 of human Prostate Specific Membrane Antigen.
Purity:
Supernatant
Form
Supplied as a liquid in PBS, pH 7.2, 0.05% BSA, 0.01% sodium azide, 50% glycerol.
Specificity:
Recognizes human Prostate Specific Membrane Antigen.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Barinka C, et al. Protein Sci 13(6):1627-35, 2004 2. The UniProt Consortium, The Universal Protein Resource (UniProt), Nucleic Acids Res. 36:D190-D195 (2008) 3. Schülke N, et al. Proc Natl Acad Sci U S A 100(22):12590-5, 2003