Technical Data
A0004
ABCA1 (ABC 1, ABC Transporter 1, ATP-binding Cassette 1 antibody, anti-ATP-binding Cassette Transporter 1, CERP, Cholesterol Efflux Regulatory Protein, HDLDT1, TGD)
Description:
Mutations in the ABC A1 gene (ATP-binding cassette transporter 1) have been reported in Tangier disease (TD). TD is an autosomal recessive disorder characterized by an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and deviations in cellular lipid trafficking. ABC A1 mediates the apo-A1 associated export of cholesterol and phospholipids from the cell. It is expressed on the plasma membrane and the Golgi complex, and is regulated by cholesterol flux. Regulation of the cholesterol flux between HDL and macrophages is competitive between ABC A1 and SR-BI.

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation Immunoflourescence, Immunocytochemistry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:250. Band representing ABC A1 is seen at ~220kD. Additional non-specific bands are seen at lower molecular weights, but do not interfere with the ABC A1 signal.
Note: It is important not to boil the sample before loading onto a gel. Boiling can cause aggregation in large proteins, resulting in the protein’s inability to enter the gel. Post-nuclear lysates of 9-cis-retinoic acid and 22R-hydroxycholesterol induced raw macrophages were used for the positive control experiments.
Immunoprecipitation: See Krimbou and/or Denis, et al. references.
Immunohistochemistry: 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Partial peptide sequence somewhere between amino acids 1100-1300 of the human ABCA1 gene.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris-citrate, pH 7.5, 0.02% sodium azide, before the addition of glycerol to 40%.
Specificity:
Recognizes human ABC A1. Species Crossreactivity: Mouse, rat and hamster.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Ning, Y. et al., (2009) Atherosclerosis 204:114–120. 1. Murthy, S., et al., J. Lipid Res. 43: 1054-1064 (2002). 2. Burgess, J.W., et al., JBC Papers in Press. 277(35): 31,318-31,326 (2002). 3. Haidar, B., et al., J. Lipid Res. 43(12): 2087-2094 (2002). 4. Terasaka, N., et al., FEBS Letters. 536: 6-11 (2003). 5. Basso, F., et al., J. Lipid Res. 44: 296-302 (2003). 6. Le Lay, S., et al., J. Lipid Res. 44(8): 1499-1507 (2003). 7. Khovidhunkit, W., et al., J. Lipid Res. 44: 1728-1736 (2003). 8. Han, J., et al., JBC Papers in Press. 276(19): 16,567-16,572 (2001). 9. Wang, N., et al., J. Clin. Invest.111(1): 99-107 (2003). 10. Escher, G., et al. J. Biol. Chem. 278(13): 11,015-11,019 (2003). 11. Feng, B., et al., J. Biol. Chem. 277(45): 43,271-43,280 (2002). 12. Fukumoto, H., et al., J. Biol. Chem. 277(50): 48,508-48,513 (2002). 13. Koldamova, R., et al., J. Biol. Chem. 278(15): 13,244-13,256 (2003). 14. Sun, Y., et al., J. Biol. Chem. 278(30): 27,688-27,694 (2003). 15. Witting, S., et al., J. Biol. Chem. 278(41): 40,121-40,127 (2003). 16. Krimbou, et al., J. Biol. Chem. 45: 839-848 (2004). 17. Denis, M., et al., J. Biol. Chem. 279(9): 7384-7394 (2004). 18. Krimbou, L., et al., J. Lipid Res. 45: 839-848 (2004). 19. Denis, M., et al., J. Biol. Chem. 279(40): 41,529-41,536 (2004). 20. Koldamova, R., et al., J. Biol. Chem. 280(6): 4079-4088 (2005). 17. Gaus, K., et al. PNAS. 100(26): 15554-15559, 2003. (human) 18. Denis, M., et al. Journal of Biological Chemistry. 279(9):7384-7394,2004. 19. Bortnick, A: Am J Physiol Lung Cell Mol Physiol 285: L869-L878, 2003. 20. Wagner, B: Promoter-Specific Roles for Liver X Receptor/Corepressor Complexes in the Regulation of ABCA1 and SREBP1 Gene Expression. Molecular And Cellular Biology, Aug.2003, pp. 5780-5789. 21. Sun, Y: Expression of Liver X Receptor Target Genes Decreases Cellular Amyloid beta Peptide Secretion. The Journal of Biological Chemistry. Vol.278, No.30 July 25,pp. 27688-27694, 2003. 22. Field, F., et al. LXR/RXR ligand activation enhances basolateral efflux of beta-sitosterol in CaCo-2 cells. Journal of Lipid Research. 45:905-913, 2004. 23. Krimbou, L., et al. Molecular interactions between apoE and ABCA1: impact on apoE lipidation. Journal of Lipid Research. 45:839-848, 2004. 24. Brown III, J., et al. Differential Expression of Cholesterol Hydroxylase in Alzheimer’s Disease. The Journal of Biological Chemistry. 279(33):34674-34681, 2004. 25. Sahoo, D., et al. ABCA1-dependent lipid effulux to apolipoprotein A-1 mediates HDL particle formation and decreases VLDL secretion from murine hepatocytes. Journal of Lipid Research. 45: 1122-1131, 2004 26. Denis, M., et al. Characterization of Oligomeric Human ATP Binding Cassette Transporter A1. The Journal of Biological Chemistry. 279(40):41529-41536, 2004. 27. Fitzgerald, M., et al. ATP-binding Cassette Transporter A1 Contains an NH(2)-terminal Signal Anchor Sequence That Translocates the Protein's First Hydrophilic Domain to the Exoplasmic Space. 276(18): 15137-15145, 2001. 28. Gaus, K., et al. Apolipoprotein A-1 interaction with plasma membrane lipid rafts controls cholesterol export from macrophages. The FASEB Journal. 10.1096/fr.03-0486fje., 2004. 29. Koldamova, R., et al. The Liver Receptor Ligand T0901317 Decreases Amyloid Beta Production in Vitro and in a Mouse Model of Alzheimer's Disease. The Journal of Biological Chemistry. 280(6): 4079-4088, 2005. 30. Ando, H., et al. Effects of Pravastatin on the Expression of ATP-Binding Cassette Transporter A1. J. Pharmacol. Exp. Ther. 311: 420-425, 2004. 31. Li. A., et al. Differential inhibition of macrophage foam-cell formation and atherosclerosis in mice by PPAR alpha alpha, B/delta, and gamma. J. Clin. Invest. 114: 1564-1576, 2004. 32. Haidar, B., et al. Apolipoprotein A-I Activates Cellular cAMP Signaling through the ABCA1 Transporter. J. Biol. Chem. 279: 9963-9969, 2004. 33. Bared, S., et al. Association of ABCA1 with Syntaxin 13 and Flotillin-1 and Enhanced Phagocytosis in Tangier Cells. Mol. Biol. Cell. 15: 5399-5407, 2004. 34. Koldamova, R., et al. The LXR ligand T0901317 decreases amyloid beta production in vitro and in a mouse model of Alzheimer's disease. J. Biol. Chem. 10.1074/jbc.M411420200, 2004. 35. Dove, D. ACAT1 Deficiency Disrupts Cholesterol Efflux and Alters Cellular Morphology in Macrophages. Arterioscler. Thromb. Vasc. Biol. 25: 128-134, 2004. 36. Agassandian, M., et al. Oxysterols Trigger ABCA1-Mediated Basolateral Surfactant Efflux. Am. J. Respir. Cell Mol. Biol. 31: 227-233, 2004. 37. O'Connell, B., et al. Cellular Physiology of Cholesterol Efflux in Vascular Endothelial cells. Circulation. 110: 2881-2888, 2004. 38. Zeng, L., et al. Sterol-responsive Element-binding Protein (SREBP) 2 Down-regulates ATP-binding Cassette Transporter A1 in Vascular Endothelial Cells: A NOVEL ROLE OF SREBP IN REGULATING CHOLESTEROL METABOLISM. J. Biol. Chem. 279: 48801-48807, 2004.1. Orso, E., et al. Transport of lipids from golgi to plasma membrane is defective in tangier disease patients and Abc1-deficient mice. Nat. Genet. 24(2): 192-196, 2000. 2. Chen, W. et al. Scavenger receptor-BI inhibits ATP-binding cassette transporter 1-mediated cholesterol efflux in macrophages. J. Biol. Chem. 275(40): 30794-30800. 20003. 3. Wang, N., et al. Specific binding of ApoA-I, enhanced cholesterol efflux, and altered plasma membrane morphology in cells expressing ABCA1. J. Biol. Chem. 275(42): 33053-58, 2000 4. Su, Y., et al. Macrophage Apolipoprotein A-I Expression Protects against Atherosclerosis in ApoE-Deficient Mice and Up-regulates ABC Transporters. Molecular Therapy. 0:1-8,2003 5. Schmid, K., et al. Transport of cholesterol across a BeWo cell monolayer: implications for net transport of sterol from maternal to fetal circulation. Journal of Lipid Research. 44: 1909-1918, 2003. 6. Khovidhunkit, W. et al. Endotoxin down-regulates ABCG5 and ABCG8 in mouse liver and ABCA1 and ABCG1 in J774 murine macrophages: defferential role of LXR. Journal of Lipid Research. 44: 1728-1736, 2003.