Technical Data
A0004-01C
ABCB5 (ATP-binding cassette, sub-family B MDR/TAP, ABCB5alpha, ABCB5beta, EST422562, ATP-binding cassette, sub-family B, member 5N: P-glycoprotein ABCB5)
Description:
ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. A number of distinct forms of ABCB5 have been shown to be expressed in various tissue types, including, but not limited to, melanocytes, melanoma cells, testis, mammary tissue, and retinal pigmented epithelium. ABCB5 is also indicated to be a metastatic melanoma progression marker.

Applications:
Suitable for use in ELISA, Western Blot. Other applications not tested.

Recommended Dilution:
Peptide ELISA: <1:128,000
Western Blot: 0.03-0.1ug/ml. Detects a band at ~80kD in lystaes of A431.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-QTQHRNTSKKAQ, from the internal region of human ABCB5 (NP_848654.3).
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes human ABCB5 at ~80kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
ABCB5-mediated doxorubicin transport and chemoresistance in human malignant melanoma., Frank NY, Margaryan A, Huang Y, Schatton T, Waaga-Gasser AM, Gasser M, Sayegh MH, Sadee W, Frank MH., Cancer Res. 2005 May 15;65(10):4320-33.