Technical Data
A0004-01K
ABCB5 (ATP-binding Cassette Sub-family B Member 5, ABCB5 P-gp, P-gp P-glycoprotein ABCB5)
Description:
ABCB5 (ATP-binding cassette, subfamily B [DR/TAP] member 5) is a 90kD (predicted MW) member of the human P-glycoprotein family of molecules. It is expressed by CD133+ pluripotent pigment stem cells, where it serves as a marker for cells that show a high incidence of polyploidy. Human ABCB5 is 812aa in length. It is possibly a five transmembrane protein with a 247aa N-terminal extracellular domain (ECD) aa1-247 and a 283aa C-terminal cytoplasmic region aa530-812. The ECD contains one ABC transporter aa2-177, while the cytoplasmic region contains a second ABC transporter aa570-808. There are two potential splice forms. One shows a seven aa substitution for aa125-812, while another possesses an alternative start site 445aa upstream of the standard site. Over aa141-247, human ABCB5 shares 64aa identity with mouse ABCB5.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:4000
Immunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ul-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to human ABCB5.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4.
Specificity:
Recognizes human ABCB5.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.