Technical Data
A0004-05B
ATP Binding Cassette subfamily G, Member 5, Control Peptide (ABCG5, Sterolin-1)
100ug
Molecular Biology Storage: -20CShipping: Blue Ice
Control peptide for A0004-05A, polyclonal antibody to ABCG5.

The ATP binding cassette (ABC) superfamily of membrane transporters is one of the largest protein classes known, and counts for numerous proteins involved in trafficking of biological molecules across membranes, host-defense mechanism to xenobiotics. The first known members were P-glycoprotein (P-gp) and multidrug resistant protein (MRP), cause multidrug resistance when transfected into drug-sensitive cells. In addition, increasing numbers of ABC proteins have recently been identified. The human ABCG1 (ABC, subfamily G, member 1) gene encodes a member of ABC superfamily that mediates the ATP-dependent translocation of variety of amphiphilic and lipophilic molecules. ABCG2 has been identified as a candidate protein responsible for cancer multidrug resistance, the overexpression of ABCG2 was found in several drug-selective cell lines. Search made of EST databases with BLAST program led to identification of several mouse and rat sequences that had high homology to ABCG2 but that appeared to encode a unique gene. ABCG3 is the most closely related to ABCG2 with 54% aa identity overall. The gene, ABCG4, produces several transcripts that differ at the 5' end and encode proteins of various lengths, the ABCG4 protein is closely related to the Drosophila's white and human ABCG1 genes, and belongs to the ABCG subfamily which are involved in cholesterol transport. ABCG5 and ABCG8 are members of the G subfamily of ABC transporters, which are predicted to contain a single magnesium-dependent ATP catalytic domain N-terminal to six transmembrane segments, mutations in either of them cause an identical phenotype which is consistent with these two gene products functioning as heterodimer. ABCG6 and ABCG7 exist in Dictyostelium species of eukaryotes.

ABCG5/sterolin-1 gene product a 651aa residue in human, and 652aa each in rat and mouse, plays an important role in the selective transport of cholesterol in and out of the enterocytes and in the selective sterol excretion by liver into bile. Strongly expressed in liver, intestine and colon. Defects in ABCG5 results increased intestinal absorption of sterols and decreased biliary excretion of dietary sterols into bile; the condition is referred as sitosterolemia.

Source:
Synthetic peptide corresponding to 13aa of human ABCG5, C-terminal. (Acession #Q53T83)

Molecular Weight:
~3kD

Applications:
Suitable for use in ELISA and Antibody Blocking. Not suitable for Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~3kD
Source: Synthetic peptide
Purity: Purified
Concentration: ~1mg/ml
Form: Supplied as a lyophilized pwder from PBS, pH7.5.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Gregory A. Graf et al (2002) J. of Clin. Inves. Vol. 110, 659-667. 2. Lu. K et al (2001) Am. J. Hum. Genet. 69 (2), 278-290. 3. Lee, M. H et al (2001) Nat. Genet. 27 (1), 79-83. 4. L. Austin Doyle (1998) PNAS Vol. 95, 15665-15670.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.