Technical Data
A0004-05D
ATP Binding Cassette subfamily G, Member 5, Control Peptide (ABCG5, Sterolin-1)
100ug
Molecular Biology Storage: -20CShipping: Blue Ice
Control peptide for A0004-05C, polyclonal antibody to ABCG5.

The ATP binding cassette (ABC) superfamily of membrane transporters is one of the largest protein classes known, and counts for numerous proteins involved in trafficking of biological molecules across membranes, host-defense mechanism to xenobiotics. The first known members were Pglycoprotein (P-gp) and multidrug resistant protein (MRP), cause multidrug resistance when transfected into drugsensitive cells. In addition, increasing numbers of ABC proteins have recently been identified. The human ABCG1 (ABC, subfamily G, member 1) gene encodes a member of ABC superfamily that mediates the ATP-dependent translocation of variety of amphiphilic and lipophilic molecules. ABCG proteins were able to transport substances across cellular membranes and against concentration gradient they require an input of energy, which requires the hydrolysis of ATP, directly or indirectly.

ABCG5 gene product a 651aa residue in human, and 652aa each in rat and mouse, plays an important role in the selective transport of cholesterol in and out of the enterocytes and in the selective sterol excretion by liver into bile. Strongly expressed in liver, intestine and colon. Defects in ABCG5 results increased intestinal absorption of sterols and decreased biliary excretion of dietary sterols into bile; the condition is referred as sitosterolemia.

Source:
Synthetic peptide corresponding to 14aa of human ABCG5, N-terminal.

Molecular Weight:
~3kD

Applications:
Suitable for use in ELISA and Antibody Blocking. Not suitable for Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~3kD
Source: Synthetic peptide
Purity: Purified
Concentration: ~1mg/ml
Form: Supplied as a lyophilized pwder from PBS, pH7.5.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Gregory A. Graf et al (2002) J. of Clin. Inves. Vol. 110, 659-667. 2. Lu. K et al (2001) Am. J. Hum. Genet. 69 (2), 278-290. 3. Lee, M. H et al (2001) Nat. Genet. 27 (1), 79-83. 4. L. Austin Doyle (1998) PNAS Vol. 95, 15665-15670.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.