Technical Data
A0004-11A
ABCG8, Rat, Control Peptide (ATP-binding Cassette Transporter 8)
100ug
Molecular Biology Storage: -20CShipping: Blue Ice
The ATP binding cassette (ABC) superfamily of membrane transporters is one of the largest protein classes known, and counts for numerous proteins involved in trafficking of biological molecules across membranes, host-defense mechanism to xenobiotics. The first known members were P-glycoprotein (P-gp) and multidrug resistant protein (MRP), cause multidrug resistance when transfected into drug-sensitive cells. In addition, increasing numbers of ABC proteins have recently been identified. The human ABCG1 (ABC, subfamily G, member 1) gene encodes a member of ABC superfamily that mediates the ATP-dependent translocation of variety of amphiphilic and lipophilic molecules. ABCG proteins were able to transport substances across cellular membranes and against concentration gradient they require an input of energy, which requires the hydrolysis of ATP, directly or indirectly.

Rat ABCG8 protein is alternatively spliced: A full length isoforms 3 (694-aa), isoforms 2 (671-aa; missing 56-77 aa, and 398-aa); isoforms 1 (672aa, missing 56-77 aa). Actual expression of various protein isoforms and their physiological relevance is not understood. ABCG8 may form heterodimers with ABCG5 or be tightly coupled to ABCG5 along a pathway regulating dietary-sterol absorption and excretion. ABCG8 protein is highly expressed in liver, intestine and colon. Defects in ABCG8 cause sitosterolemia.

Source:
15-aa peptide from Rat ABCG8, conjugated to KLH ~within N-terminus, Cytoplasmic

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: Control peptide can be used to coat ELISA plates at 1ug/ml and detected with antibodies (0.5-1ug/ml for affinity pure).

Control peptide, because of its low mol. Wt (<3 kD), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10ug control peptide per 1ug of affinity purified IgG or 1ul antiserum) to confirm antibody specifity.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity: Purified by HPLC.
Concentration: ~1mg/ml
Form: Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity: Rat control peptide is 100% conserved in rat ABCG-8 isoforms 1-3. It is 65% in mouse ABCG8 and no appreciable conservation in human ABCG8. Antibody reactivity and actual expression of various protein isoforms and their physiological relevance is not understood. Antibody cross-reactivity in various species has not been studied.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1.) Lu. K et al (2001) Am. J. Hum. Genet. 69 (2), 278-290; 2.) Berge, K. E et al (2000) Science 290 (5497), 1771-1775; 3.) Lee, M. H et al (2001) Nat. Genet. 27 (1), 79-83; 4.) L. Austin Doyle (1998) PNAS Vol. 95, 15665-15670.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.