Technical Data
ABIN-1 (A-20 binding Inhibitor of Nuclear Factor-kappaB (NF-kB) Activation)
A20-binding inhibitor of NFkB activation (ABIN-1) is involved in NFkB-dependent gene expression and plays an important role in numerous cellular processes including stress responses, inflammation and cell proliferation. ABIN-1 increases cell surface CD4 (T4) antigen expression, and it interacts with HIV-1 matrix protein and is packaged into virions. Overexpression of ABIN-1can .inhibit viral replication. ABIN-1 may also regulate matrix nuclear localization, both nuclear import of PIC (Preintegration complex) and export of GAG polyprotein and viral genomic RNA during virion production. ABIN-1 is strongly expressed in peripheral blood lymphocytes, spleen and skeletal muscle, and is weakly expressed in the brain.

Suitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications have not been tested.

Recommended Dilutions:
ELISA (Immunogen): 0.1-1ug/ml
Immunoprecipitatio (Human): 7-10ug/test
Western Blot (Human): 1-3ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
HEK-293 cell lysate.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG2a,k6A2Affinity Purified
100ug-20CBlue IceHumanMouse
Synthetic peptide corresponding to the N-terminal region of the human, mouse and rat ABIN-1 protein.
Purified by Protein A chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Recognizes the ~80kD human ABIN-1 protein. Bands of unknown origin at ~180kD and >250kD were observed in Western Blots using HEK-293 cell lysates. Species Sequence Homology: mouse and rat 100%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Fukushi M, et al. FEBS Lett 442:83-88, 1999. 2. Heyninck K, et al. FEBS Lett 563:135-140, 2003.