Technical Data
A0007-34G
ABI1 (Abl Interactor 1, Abelson Interactor 1, Abi-1, Spectrin SH3 Domain-binding Protein 1, Eps8 SH3 Domain-binding Protein, Eps8-binding Protein, e3B1, Nap1-binding Protein, Nap1BP, Abl-binding Protein 4, AblBP4, SSH3BP1)
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Technical Data |
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A0007-34G |
ABI1 (Abl Interactor 1, Abelson Interactor 1, Abi-1, Spectrin SH3 Domain-binding Protein 1, Eps8 SH3 Domain-binding Protein, Eps8-binding Protein, e3B1, Nap1-binding Protein, Nap1BP, Abl-binding Protein 4, AblBP4, SSH3BP1) |
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Description: ABI1 is a human homolog of mouse Abl-interactor-1 (Abi1), mapped on 10p11.2. ABI1 participate in the transduction of signals from Ras to Rac by regulating Rac-specific guanine nucleotide exchange factor (GEF) activities. ABI1 dramatically promoted ABL1-mediated tyrosine phosphorylation of MENA, but not of other substrates. Abi-1 regulates c-Abl-mediated phosphorylation of Mena by interacting with both proteins. ABI1 plays a role in the leukemogenesis by translocating to MLL. Applications: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml Immunohistochemistry (frozen/paraffin): 0.5-1ug/ml Immunocytochemistry: 0.5-1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH20. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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