Technical Data
ABI1 (Abl Interactor 1, Abelson Interactor 1, Abi-1, Spectrin SH3 Domain-binding Protein 1, Eps8 SH3 Domain-binding Protein, Eps8-binding Protein, e3B1, Nap1-binding Protein, Nap1BP, Abl-binding Protein 4, AblBP4, SSH3BP1)
ABI1 is a human homolog of mouse Abl-interactor-1 (Abi1), mapped on 10p11.2. ABI1 participate in the transduction of signals from Ras to Rac by regulating Rac-specific guanine nucleotide exchange factor (GEF) activities. ABI1 dramatically promoted ABL1-mediated tyrosine phosphorylation of MENA, but not of other substrates. Abi-1 regulates c-Abl-mediated phosphorylation of Mena by interacting with both proteins. ABI1 plays a role in the leukemogenesis by translocating to MLL.

Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml
Immunohistochemistry (frozen/paraffin): 0.5-1ug/ml
Immunocytochemistry: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile dH20. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
As reported
Synthetic peptide corresponding to a sequence at the C-terminal of human ABI-1, different from the related rat and mouse sequence by single amino acid
Purified by immunoaffinity chromatography.
Supplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with sterile dH2O.
Recognizes human ABI1. Species Cross-Reactivity: mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.