Technical Data
ACADM (Acyl-Coenzyme A Dehydrogenase, C-4 to C-12 Straight Chain, ACAD1, MCAD, MCADH, Medium-chain Specific Acyl-CoA Dehydrogenase, Mitochondrial Precursor)
ACADM protein is a medium chain specific (C4 to C12 straight chain) acyl Coenzyme A dehydrogenase. The enzyme catalyzes the initial step of the mitochondrial fatty acid beta oxidation pathway. ACADM expression is induced during periods of fasting, when reliance on fatty acids for energy is increased. Clinical phenotypes are associated with ACADM hereditary deficiency.

Cellular Localization: Mitochondrial

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:32000.
Western Blot: 0.01-0.1ug/ml. Predicted molecular weight 48kD. Detects a band of ~ 45kD
Optimal dilutions to be determined by the researcher.

Storage and Stability:

May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanGoat
Synthetic peptide: RLIVAREHIDKYKN representing the C-terminus of the human protein (residues 408-421), according to NP_000007.
Purified by immunoaffinity chromatography.
Supplied as a liquid in TBS, pH 7.2, 0.5% BSA.
Species Crossreactivity: Crossreacts with Human. Expected to crossreact with Mouse (100% identity with immunogen) and Rat (100% identity with immunogen) due to sequence homology.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Matsubara Y et al. Molecular cloning of cDNAs encoding rat and human medium-chain acyl-CoA dehydrogenase and assignment of the gene to human chromosome 1. Proc Natl Acad Sci U S A 83:6543-7 (1986).