Technical Data
ACSL5 (A-CoA Synthetase Long-chain Family Member 5, HGNC:16526, ACS2, ACS5, FACL5, Fatty-acid-Coenzyme A Ligase, Long-chain 5, Long-chain Acyl-CoA Synthetase 5)
The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in uterus and spleen, and in trace amounts in normal brain, but has markedly increased levels in malignant gliomas. This gene functions in mediating fatty acid-induced glioma cell growth. Three transcript variants encoding two different isoforms have been found for this gene.

Positive Control: Human Spleen lysate

Suitable for use in ELISA (peptide) and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA (peptide): 1:16,000
Western Blot: 1-3ug/ml. ~ 75kD band observed in Human Spleen lysates (calculated MW of 76.0kD according to NP_976313.1and NP_976314.1).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabAffinity Purified
100ug-20CBlue IceHumanGoat
C-RTQIDSLYEHIQDC TerminusNP_057318.2; NP_976313.1; NP_976314.1. Homology: Human
Purified by immunoaffinity chromatography.
Supplied as a liquid in Tris-saline, pH 7.2. 0.5% BSA, 0.02% sodium azide.
Species Crossreactivity: Human. Other species not tested.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Obermuller N, Keith M, Kopitz J, Autschbach F, Schirmacher P, Gassler N. Coeliac disease is associated with impaired expression of acyl-CoA-synthetase 5.Int J Colorectal Dis. 2005 Apr 5; [Epub ahead of print] PMID: 1580983